OsmiRNA156 is a free miRNA that cleaves the mRNA transcript of the OsSPL14 gene. PCR amplification was carried out using PROMEGA kit Madison Company USA. PCR master mix was prepared for a total of 24 samples having18 μl for each sample. The individual sample reaction contains 12.24μl of water 2Mm MgCl2, taq buffer of 0.4 μl. 0.2 μl for each forward and reverse primer of 20Mm 0.4 of DNTPS and 0.16 of tag polymerase. PCR conditions were set for 40 cycles set at 94°C for 2min, followed by 30 seconds 94°C, 30 sec on 55 °C and 72°C for 30 sec, and final extension of 72°C for 10 min. During the last three years, rice production has reached to a record level. During 1940–1960s, green revolution led to an expansion in creation of agro-based industry especially in devolving countries, primarily through progress in research and innovation .
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- Morphological evaluation of genotypes showed highest number of tillers for Basmati 370, the lowest was recorded for Line 77.
- It is a physiological process of continuous underground branching of compact node joints of the primary shoot.
- The SPL genes are regulated by microRNA miR156, and OsSPL16 of OsmiR156 was shown to repress OsSPL16 transcription in young panicles .
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Supporting information
The binding of miR156 to the seed sequence of SPL-14 indicates the amount of SQUAMOSA PROMOTER BINDING PROTEIN-LIKE 14 gene expressions. Previous studies reported that microRNA imperfectly binds to seed sequence . We also identified some lines that showed low grains per panicle, so we suggested that it might be some other genes regulating grain per panicle. Similarly, the grain yield per plant of Line-77, Line-20, and Line-48 also had low grain yield per plant.
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